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Regular use of exfoliating beads beneficial

Mickey McDonnell – Honeywell, United States

Desquamation is the natural process of old, dead cells falling off the surface of our skin. It is estimated that each of us sheds as many as a billion cells each day.1,2 Desquamation has been postulated to evolve in order to remove the cells damaged by airborne toxins and eliminate waste from inside the body, such as excess iron.2 Exfoliation hastens the desquamation process and exposes skin cells with a brighter appearance and a tighter feel. Chemicals can be used to reduce the cohesion between the cells and promote sloughing, but the physical action of exfoliating beads may be a safer way to achieve the same result.

Exfoliating beads have a long history in cosmetic applications, but we are unaware of previous studies to substantiate their effectiveness. One may expect that an individual application of scrubbing beads promotes desquamation, but we wanted to discover if their regular use leads to definite, quantifiable changes in the skin.

To better understand exfoliation we need an appreciation of the basic physiology of the epidermis, the outer layer of the skin. The cells that desquamate come from the outermost layer of cells, the stratum corneum (SC). The cells of this layer are no longer living and are often described as protein bricks in a waxy mortar. The number of layers of cells in the SC varies substantially depending on the part of the body, with a maximum number of layers on the heel and a minimum on the genitals.3

Typically the face and arms have 10 to 20 layers. Most studies indicate that the number of layers does not change with age or vary between men and women.4 The cells of the SC have lost their nuclei and are filled primarily with the structural protein keratin and small amounts of melanin pigments that absorb light and degraded protein fragments that retain moisture, the NMFs or natural moisturising factors. The mortar is made of sheets of lipids that make the skin water repellent. These hydrophobic molecules cause water to bead up and run off our skin when we shower. Protein links, the desmosomes, run through the intercellular lamellar lipid layer to bind the cells together. Multiple enzymes assist in the degradation of these links before the cells desquamate. Low humidity hinders the degradation of the desmosomes and leads to flaky skin.

Below the SC is the vital epidermis, or Malpighi layer, which is made up of living cells. At the innermost side one layer of cells divides. As these cells progress up through the vital epidermis they undergo a progression of changes before entering the SC. Desquamation can be seen as the final step in the maturation of a skin cell.

The best way to measure desquamation is not obvious. Surface cells can easily be observed by pulling a strip of adhesive tape off your finger. Your unaided eye sees an impression of the fingerprint, but a low powered microscope reveals that the print is actually lines of small scaly cells stuck to the tape. This technique verifies that cells have been removed, but it is not the consequence of the natural process of desquamation. To find the rate of natural desquamation cylindrical chambers with a radius and height of about 1 cm have been glued to the skin on various parts of the body to collect the desquamating cells over 48 hours.1 A much simpler test is to stain the skin with a dansyl chloride dye and monitor its presence by its UV fluorescence.5

Following the dyeing process with skin biopsies showed that with sufficient application time the dansyl chloride stains the whole SC layer but does not enter the vital epidermis.5 After applying the stain the level of florescence decreased linearly during the next one to three weeks, and biopsies show a reduction in the number of stained layers in the SC proportional to the decrease in the optical signal.6 The duration from the initial staining to the loss of detectable fluorescence is called the SC turnover time. The turnover time for different parts of the body correlated well with the number of cells collected per unit area by either natural or forced exfoliation.4 Because of its simplicity to monitor, the SC turnover time has become the most commonly measured indicator of desquamation rate. One study reported turnover times for different parts of the body varying from 8.5 days for the forehead to 17.2 days for the lower arm with 12.6 days for the upper arm,7 although there is substantial variation among the results reported from different laboratories.8

Both natural and synthetic exfoliating beads are used in cosmetic applications. Beads from natural sources are more susceptible to bacterial contamination. Synthetic beads are often made of polyethylene or oxidised polyethylene with a wide selection of particle sizes and surface properties. Depending on the application, the average diameters range from about 150 to 600 µm.

Test methodology

For this study we selected Asensa SC 232 which is a hard, high density oxidised polyethylene bead recommended for body/facial scrubs that has an average particle diameter of 325 µm. Figure 1 shows an electron micrograph illustrating the irregular shapes and slightly rounded edges of the particles. For this study beads were formulated at the 10% level in a moderately abrasive hand/body cleanser (Fig. 2). A second formulation, identical in all respects except it contained no scrubbing beads, was prepared as one control. No treatment was used as a second control. Both formulations were coded to mask their identity.

To find out if routine use of such a cleanser affects desquamation, we established a contract with an independent testing clinic to test the products. The study was conducted in accordance with the intent and purpose of Good Clinical Practice regulations described in the CFR Title 21, Parts 50, 56 and 312 and/or the Declaration of Helsinki. A pool of 11 Caucasian women between the ages of 28 and 68 were enrolled in the study. Ten subjects with an average age of 49 completed the study. All panellists were interviewed and examined to determine that they had no skin conditions which could interfere with the study. Three squares of one inch length were marked on the upper outer arms of each subject. The centre square on each arm was selected as a no-treatment control area. Two of the remaining four sites were randomly assigned to the Asensa SC 232 scrub and the formulation with no beads. The other two sites were used in other studies.

Since we wanted to mimic regular use of exfoliating products, the women were taught how to apply the scrub to its assigned site twice daily for a two-week period of acclimatisation. They were instructed to avoid washing their arms or swimming for four hours after applications, to refrain from using scrubs, loofah sponges or wash cloths on their arms and to use no moisturisers, sunscreens or other toiletries on their arms. One subject was removed from the study when she developed a rash. The rash covered both control and treatment sites and was suspected to be caused by the soap specified by the test clinic to be used during the trial. After two weeks of use, on a Monday morning, the six sites of the remaining 10 subjects were stained with dansyl chloride for five hours and examined under a long wave UV lamp to verify that each site was uniformly and fully stained. Each site was evaluated by a trained clinical technician on a score of 0 to 3 in measurement units of 0.5 where:

ABSTRACT
Exfoliating beads have a long history in cosmetics, but we are unaware of previous studies on the effect of their regular use. A clinical study with 10 subjects shows that regular exfoliation leads to a reduction in the cell turnover time of the stratum corneum (SC) by about 15% (significant at the 95% level). Standard models of skin function interpret increased cell turnover time as increased desquamation. The regularly exfoliated skin also had a significantly increased uptake of dansyl chloride dye. Higher stain uptake accompanying the increase in desquamation suggests that regular exfoliation promotes addition of new cells to the SC. The study acclimatised the test panel to the exfoliant treatment for two weeks prior to the measurements to minimise the chance that results could be attributed to a shift in treatment procedures

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Upcoming Events

in-cosmetics Global

Paris Expo Porte de Versailles
16th – 18th April 2024

NYSCC Suppliers' Day 2024

Javits Center, New York
1st - 2nd May 2024

9th Anti-Ageing Skin Care Conference

Royal College of Physicians, London
25th - 26th June 2024

in-cosmetics Korea 2024

Hall C, Coex, Seoul, South Korea
24th - 26th July 2024

in-cosmetics Latin America 2024

São Paulo, Brazil
25th - 26th September 2024

IFSCC 2024

Recanto das Cataratas Thermas Resort, Iguazu Falls, Brazil
14th - 17th October 2024

Access the latest issue of Personal Care Magazine on your mobile device together with an archive of back issues.

Download the FREE Personal Care Magazine app from your device's App store

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